HPLC Column, RP C4 Butyl, Wide Pore, 10um, 4.0mm ID x 150mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase combines moderate hydrophobic retention with a 300 Å pore structure that improves analyte access for larger molecules. The C4 chemistry is particularly well suited for proteins, peptides, enzymes, and other biomolecules that require efficient mass transfer and reduced hydrophobic interaction compared to more retentive reversed-phase phases.
68004-15P-4-3M is a 150 mm x 4.0 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The 150 mm column length provides increased resolving power and peak capacity compared to shorter column formats, making it an excellent choice for biomolecule characterization, impurity profiling, and method optimization. The 10 µm particle size supports higher flow rates with lower operating backpressure, while the 4.0 mm ID format is commonly selected for legacy methods and conventional HPLC systems and typically operates at approximately 0.8–1.5 mL/min.
This USP L26 column is designed for the separation and characterization of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. The combination of fully endcapped C4 chemistry, high-purity silica, wide pore architecture, and increased column length provides improved resolution, excellent analyte recovery, and reliable chromatographic performance for demanding bioanalytical and biomolecule-focused applications. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
USP L26 classification
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
300 Å wide pore structure optimized for large molecules
High-purity silica support
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Enhanced resolution from 150 mm column length
Excellent for protein and peptide separations
Typical Applications
Reversed-phase HPLC
USP L26 methods
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
Biomolecule separations
Impurity profiling
Method development
Large molecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 150 mm
Length: 150 mm
Recommended Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This item is a Special Order Item manufactured specifically to customer requirements.
Special Order Items cannot be canceled or returned once an order has been placed.
Manufactured upon receipt of order.
May have extended lead times compared to stocked products.
Important Notes
Designed for reversed-phase chromatography of proteins and other large molecules
Wide pore structure improves analyte accessibility and recovery
Provides lower hydrophobic retention than comparable C8 phases
Larger particle size reduces operating backpressure
Longer column length enhances separation of closely related biomolecules
Compatible with conventional HPLC systems
Alternative for many USP L26 methods
Special Order Item